Journal: Experimental and Therapeutic Medicine

Article Title: KDM6A suppresses hepatocellular carcinoma cell proliferation by negatively regulating the TGF-β/SMAD signaling pathway

doi: 10.3892/etm.2020.9000

Figure Lengend Snippet: KDM6A negatively regulates the TGF-β/SMAD signaling pathway. (A) The effect of KDM6A overexpression on the expression of TGF-β, p-smad2, p-smad4, Ki67 and PCNA in Huh7 and LM3 cells was (A) determined by western blotting and (B) semi-quantified. The effect of KDM6A knockdown on the expression of TGF-β, p-smad2, p-smad4, Ki67 and PCNA in YY-8103 and SNU-398 was (C) determined by western blotting and (D) semi-quantified. ** P<0.01 and *** P<0.001 vs. vector or SCR. KDM6A, lysine demethylase 6A; TGF-β, transforming growth factor-β; p, phosphorylated; PCNA, proliferating cell nuclear antigen; SCR, scrambled; sh, short hairpin RNA; ns, not significant.

Article Snippet: Subsequently, the membranes were incubated at 4˚C overnight with the following primary antibodies: Anti-KDM6A (1:1,000; cat. no. orb333886; Biorbyt Ltd.), anti-transforming growth factor (TGF)-β (1:1,000; cat. no. 3711; Cell Signaling Technology, Inc.), anti-phosphorylated (p)-smad2 (1:1,000; cat. no. 18338; Cell Signaling Technology, Inc.), anti-Smad2 (1:1,000; cat. no. 12570-1-AP; ProteinTech Group, Inc.), anti-p-smad4 (1:1,000; cat. no. 10231-8-AP; ProteinTech Group, Inc.), anti-smad4 (1:1,000; cat. no. 10231-1-AP; ProteinTech Group, Inc.), anti-proliferating cell nuclear antigen (PCNA; 1:1,000; cat. no. 10205-2-AP; ProteinTech Group, Inc.), anti-Ki67 (1:1,000; cat. no. 27309-1-AP; ProteinTech Group, Inc.), anti-GAPDH (1:1,000; cat. no. 10494-1-AP; ProteinTech Group, Inc.) and anti-Flag (1:4,000; cat. no. F7425; Sigma-Aldrich; Merck KGaA).

Techniques: Over Expression, Expressing, Western Blot, Plasmid Preparation, shRNA

Journal: Materials Today Bio

Article Title: Bioactive recombinant human elastin gel for reconstructing elastic fibers and rejuvenating endogenously aged skin

doi: 10.1016/j.mtbio.2025.102270

Figure Lengend Snippet: Effects of different treatments on TGF-β1, Smad3, MMP-2, and elastin expression in skin tissue. Representative immunofluorescence staining images of (a) TGF-β1, (b) Smad3, (c) MMP-2, and (d) elastin (green) with DAPI nuclear counterstaining (blue) in the Blank, Model, VE, RHC gel, and RHE gel groups. Scale bars = 100 μm. Quantitative analysis of fluorescence intensity is shown in (e–h). Data are presented as mean ± SD (n = 3). Statistical significance: ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The sections were then incubated with primary antibodies against elastin (Thermo Fisher Scientific, PA5-89281), MMP-2 (Proteintech, 10373-2-AP), TGF-β1 (Proteintech, 26155-1-AP), and Smad3 (Proteintech, 66516-1-lg), followed by fluorophore-conjugated secondary antibodies.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence